ColiIndex-51 MPN Plate

The ColiIndex-51 MPN Plate is a sterile 51-well quantitative plate designed for use with Coliplate® reagents to deliver accurate and reliable microbial water testing. With a capacity to analyze a full 100 mL water sample without dilution, each plate can detect up to 200 MPN of target bacteria, making it a practical solution for routine monitoring and compliance testing.

Product Overview

The ColiIndex-51 plate is engineered for precision and ease of use, allowing laboratories to perform quantitative microbial testing through the Most Probable Number (MPN) method. By mixing reagents with the water sample, loading the plate, and sealing it using a programmable quantitative sealer, laboratories can efficiently generate reproducible results. After incubation, simply record the number of positive large and small wells and use the provided MPN table to determine bacterial concentrations.

Quality & Sterility

Each batch of ColiIndex-51 plates is sterilized with ethylene oxide gas. Sterility reports can be downloaded from the official Quetest website, ensuring traceability and confidence in every test.

Material Composition

• Blister cover: Polyethylene

• Back sealing film: Multi-layer composite aluminum foil with adhesive

• Manufactured with a non-fluorescent process, ensuring no background fluorescence under 366 nm UV light for accurate fluorescence-based assays.

Operating Instructions

1. Hold the 51-well plate upright with openings facing up and the well surface toward your palm.

2. Gently bend the plate and peel back the foil backing without inserting fingers inside to avoid contamination.

3. Pour 100 mL of the water sample into the plate, avoiding contact between the bottle mouth and the plate. Tap lightly to release bubbles.

4. Place the plate face-down in the rubber tray and insert it into the programmable sealer. Retrieve the sealed plate from the back end.

5. Incubate according to Coliplate reagent guidelines, then record positive wells and consult the MPN table for results.

Notes & Precautions

• Avoid applying excessive force when peeling back the foil backing to prevent damage.

• Replace any plate found to be damaged before or during use.

• Used plates must be disposed of in biohazard waste bags and sterilized at 121 °C under high-pressure steam.

• A few unfilled wells will not affect results; only filled wells are considered in the calculation.

• Always incubate with wells facing upward; avoid excessive stacking to maintain proper airflow.

Compliance & Standards

The ColiIndex-51 MPN Plate complies with:

• HJ 1001-2018 – Water Quality – Determination of Total Coliforms, Fecal Coliforms, and E. coli – Enzyme Substrate Method.